Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L.

Authors

  • José Antonio Moreno Serrano E.T.S.I. de Montes, Forestal y del Medio Natural. E.T.S. Ingenieros Agronómica, Alimentaria y de Biosistemas. Departamento de Biotecnología y Biología Vegetal. UPM. Madrid. España.
  • César Pérez Ruíz E.T.S.I. de Montes, Forestal y del Medio Natural. E.T.S. Ingenieros Agronómica, Alimentaria y de Biosistemas. Departamento de Biotecnología y Biología Vegetal. UPM. Madrid. España.
  • Ivonne Moreno Fierro E.T.S.I. de Montes, Forestal y del Medio Natural. E.T.S. Ingenieros Agronómica, Alimentaria y de Biosistemas. Departamento de Biotecnología y Biología Vegetal. UPM. Madrid. España.
  • Jorge Moreno Fierro University of Bedfordshire. Vicarage St. Luton. LU1 3JU. United Kingdom

Keywords:

plant growth, germination, organogenesis, callogenesis

Abstract

This paper describes the effect of the various plant growth regulators of the culture medium on morphogenic processes in vitro in Cinchona officinalis L, a highly vulnerable species from southern Ecuador. To do this, different concentrations of NaOCl were used in combination with different immersion times for seed disinfection; for seed germination in vitro GA3 was added to the MS basal culture medium in different concentrations, and for morphogenic processes in vitro, different concentrations of auxins and cytokinins were combined. The decrease in the contamination rate was with high concentrations of NaOCl and an increase in the germination rate in 45 days with the addition of 1.0 mg L-1 GA3 to the culture medium the hormonal combination of 0.5 mg L-1 NAA + 2.5 mg L-1 BAP showed a high rate of shoot proliferation and with 1.0 mg L-1 NAA a high number of roots was obtained. In the callogenesis phase, the best results were obtained with 1.0 mg L-1 2,4-D + 0.5 mg L-1 BAP for callus proliferation. In vitro propagation protocols were generated in Cinchona officinalis L, for the preservation and conservation of the species.

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Published

30-06-2019

Issue

Section

Natural resources and environment