In vitro efficacy testing of citronella grass oil against Tritrichomonas foetus trophozoites

Authors

  • María Rosana Ramirez Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Universitario de Ciencias de la Salud, Fundación H. A. Barceló. Buenos Aires. Argentina
  • Estefanía Sereno Bruno Universidad Nacional de La Pampa. Facultad de Ciencias Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). La Pampa. Argentina
  • Debora Manuale Universidad Nacional del Litoral. Instituto de Investigaciones en Catálisis y Petroquímica. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Santa Fe. Argentina
  • Juan Carlos Yori Universidad Nacional del Litoral. Instituto de Investigaciones en Catálisis y Petroquímica. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Santa Fe. Argentina
  • Jorge Oyhenart Universidad Nacional de La Pampa. Facultad de Ciencias Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). La Pampa. Argentina

Keywords:

disease, venereal, trichomonas, trichomoniasis, antimicrobial, dose-response

Abstract

Tritrichomonas foetus, a sexually transmitted parasitic protozoan, causes abortion in cattle. Nitroimidazoles, such as metronidazole, treat bovine trichomonosis, but their use is precluded. Plant extracts might have antiparasitic effects. This study aimed to assess Cymbopogon nardus oil activity against T. foetus as an alternative to metronidazole. T. foetus trophozoites were incubated in culture medium containing serial dilutions of C. nardus oil. Cytotoxicity was assessed 24 hours later. C. nardus oil killed T. foetus cells. Half maximal effective concentration (EC50) was 0.4 μg/mL. These findings suggest that C. nardus oil could be exploited for discovery and compound isolation of plant derived phytopharmaceuticals for bovine trichomoniasis and other protozoan diseases.

Highlights:

  • Tritrichomonas foetus causes abortion in cattle.
  • Effective drugs are available but treatment is precluded.
  • Cymbopogon nardus oil kills foetus trophozoites in a dose-dependent manner.
  • Plant derived material could be used for bovine trichomonosis and other protozoan diseases.

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Published

13-08-2024

Issue

In press

Section

Production and animal health

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