FTIR Analysis of colagen preservation and possible contamination in Quaternary bones samples from the Pampas

Authors

  • Paula Vitale Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano, Facultad de Ciencias Sociales. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires, Facultad de Ingeniería. Universidad Nacional del Centro de la Provincia de Buenos Aires
  • Julia Tasca Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires, Facultad de Ingeniería. Universidad Nacional del Centro de la Provincia de Buenos Aires
  • Marcela Bax Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano, Facultad de Ciencias Sociales. Universidad Nacional del Centro de la Provincia de Buenos Aires
  • Ariadna Flores Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano, Facultad de Ciencias Sociales. Universidad Nacional del Centro de la Provincia de Buenos Aires
  • Gustavo G. Politis Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Arqueológicas y Paleontológicas del Cuaternario Pampeano, Facultad de Ciencias Sociales. Universidad Nacional del Centro de la Provincia de Buenos Aires
  • Luciano Valenzuela Laboratorio de Ecología Evolutiva Humana. Núcleo de Estudios Interdisciplinarios de Poblaciones Humanas de Patagonia Austral, Facultad de Ciencias Sociales. Universidad Nacional del Centro de la Provincia de Buenos Aires

Keywords:

Bone protein, Collagen contamination, FTIR

Abstract

This paper analyzes FTIR (infrared spectroscopy by Fourier transform) spectra of type I collagen samples extracted from modern and archaeological bones of guanaco (Lama guanicoe) and Pampas deer (Ozotoceros bezoarticus) with varying degrees of protein conservation and, in some cases, external organic input. C:N atomic ratio, C%, N%, and collagen yield were determined. Four transmittance ratios from the spectra were analyzed: Amide III/Amide I, Amide III/Proline, Proline/Amide I, 1080 cm-1/Amide I, which we propose be used for determining collagen quality. Since FTIR analysis is very versatile, it can be used as a simple decision-making tool, it is inexpensive, rapid, and available, we argue that establishing quality parameters with this method would allow us to determine collagen preservation in archaeological samples before sending them for isotopic and radiometric analyses and select the best samples.

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Anales de Arquelogía y Etnología 74 (2) (2019)

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Published

30-12-2019

How to Cite

Vitale, P., Tasca, J. ., Bax, M., Ariadna Flores, Politis, G. G. ., & Valenzuela, L. (2019). FTIR Analysis of colagen preservation and possible contamination in Quaternary bones samples from the Pampas. nales e Arqueología Etnología, 74(2), 169–189. etrieved from https://revistas.uncu.edu.ar/ojs3/index.php/analarqueyetno/article/view/3737

Issue

Vol. 74 No. 2 (2019)

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Dossier

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