First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina

Autores/as

  • Julia A. Martino Instituto Nacional de Tecnología Agropecuaria. Instituto de Patología Vegetal (Argentina). 11 de Septiembre 4755 (ex-camino 60 cuadras km 5,5). X5020ICA. Córdoba. Argentina
  • Liliana del Valle Di Feo Instituto Nacional de Tecnología Agropecuaria. Instituto de Patología Vegetal (Argentina). 11 de Septiembre 4755 (ex-camino 60 cuadras km 5,5). X5020ICA. Córdoba. Argentina.
  • Mauro Paccioretti Instituto Nacional de Tecnología Agropecuaria. Instituto de Patología Vegetal (Argentina). 11 de Septiembre 4755 (ex-camino 60 cuadras km 5,5). X5020ICA. Córdoba. Argentina
  • Clara Adriana Contardi Instituto Nacional de Tecnología Agropecuaria. Agencia de Extensión Rural Luján de Cuyo. Mendoza. Argentina
  • Miguel A. Sanchez Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (Argentina). 11 de Septiembre 4755 (ex-camino 60 cuadras km 5,5). X5020ICA. Córdoba. Argentina
  • Leandro I. Ortega Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales (Argentina) 11 de Septiembre 4755 (ex-camino 60 cuadras km 5,5). X5020ICA. Córdoba. Argentina
  • Silvina E. Pastor Instituto Nacional de Tecnología Agropecuaria. Instituto de Patología Vegetal (Argentina). 11 de Septiembre 4755 (ex-camino 60 cuadras km 5,5). X5020ICA. Córdoba. Argentina.

DOI:

https://doi.org/10.48162/rev.39.027

Palabras clave:

Ipomoea batatas, podredumbre de raíz, Chalara elegans, enfermedad fúngica en batata

Resumen

Symptomatic sweet potato cv Arapey INIA samples were collected from a commercial production field in Colonia Molina, Guaymallén department, Mendoza province, Argentina. They showed dark rounded lesions, sometimes coalescing with white granular mycelium. Fungus was obtained from symptomatic sweet potatoes, which represented the generalized infection that affected the crop. They were seeded in PDA with streptomycin sulfate and incubated for seven days at 21°C, alternating white/black (UV400nm) light. Observations with an optical microscope revealed the presence of hyaline, not septated, cylindrical endoconidia with rounded ends. They were 8-16 μm length and 4–6 μm width. Phialides were 43-46 μm length, rounded bases (7-9 μm width) and tapering to the neck´s tip (4-6 μm width). Brown chlamydospores (aleuriospores), 9-13 μm length and 8-12 μm width, in chains of 2-8 spores were observed. For molecular identification, total genomic DNA was extracted. ITS fragment of 565 pb was amplified using ITS5/ITS4 primers and sequenced. The sequence indicated 99% identity with Berkeleyomyces basicola (synonymous: Thielaviopsis basicola). This was deposited in GenBank as (KX580957) (CBS: C430.74, Gen Bank accession number AF275482.1). This is the first report of B. basicola in sweet potato in Argentina, a potential threat to storage root yields.

Highlights:

  • Sweet potato black root rot, new disease in Argentina.
  • First report of Berkeleyomyces basicola  causing black root rot on sweet potato in Mendoza, Argentina.

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Publicado

07-07-2021

Cómo citar

Martino, J. A., Di Feo, L. del V., Paccioretti, M., Contardi, C. A., Sanchez, M. A., Ortega, L. I., & Pastor, S. E. (2021). First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina. Revista De La Facultad De Ciencias Agrarias UNCuyo, 53(1), 283–287. https://doi.org/10.48162/rev.39.027

Número

Sección

Protección vegetal