PCR identification of lactic acid bacteria populations in corn silage inoculated with lyophilised or activated Lactobacillus buchneri

Authors

  • Ana Paula Maia dos Santos Alagoas State University. Departament of Animal Science. Rod. BR-316, Km 87.5. s/n. 57500-000. Santana do Ipanema. Alagoas. Brazil https://orcid.org/0000-0002-6023-7080
  • Edson Mauro Santos Federal University of Paraíba. Department of Animal Science. Rod. BR-079, 58397- 000. Areia. Paraíba. Brazil
  • Juliana Silva de Oliveira Federal University of Paraíba. Department of Animal Science. Rod. BR-079, 58397- 000. Areia. Paraíba. Brazil
  • Gherman Garcia Leal de Araújo Brazilian Agricultural Research Corporation. Embrapa Tropical Semiarid, Rod. BR- 428. Km 152. s/n, 56302970. Petrolina. Pernambuco. Brazil https://orcid.org/0000-0001-9605-1096
  • Anderson de Moura Zanine Federal University of Maranhão. Department of Animal Science. Rodovia BR 222. km 4. s/n. 65500-000. Chapadinha. Maranhão. Brazil https://orcid.org/0000-0003-0100-3652
  • Ricardo Martins Araújo Pinho Federal Institute of Education. Science and Technology of Ceara. Department of Animal Science. Rod. BR-292. s/n. Gisélia Pinheiro. 63155-500. Crato. Ceará. Brazil https://orcid.org/0000-0002-2816-1610
  • Thiago Vinícius Costa do Nascimento Federal University of Sergipe. Department of Veterinary. Nossa Senhora da Glória. 49680-000. Sergipe. Brazil https://orcid.org/0000-0002-9569-3107
  • Alexandre Fernandes Perazzo Federal University of Piaui. Department of Planning and Agricultural Policy. Campus Universitário Ministro Petrônio Portella. Bairro Ininga. 64049-550. Teresina. Piauí. Brazil
  • Daniele de Jesus Ferreira Federal University of Maranhão. Department of Animal Science. Rodovia BR 222. km 4. s/n. 65500-000. Chapadinha. Maranhão. Brazil
  • Alberto Jefferson da Silva Macedo Federal University of Viçosa. Department of Animal Science. Av. P H Rolfs. s/n - Campus Universitário. 36570-900. Viçosa. Minas Gerais. Brazil
  • Francisco Naysson de Sousa Santos Federal University of Maranhão. Department of Animal Science. Rodovia BR 222. km 4. s/n. 65500-000. Chapadinha. Maranhão. Brazil https://orcid.org/0000-0001-6968-1738

DOI:

https://doi.org/10.48162/rev.39.101

Keywords:

fermentation, inoculant, lactic acid bacteria, polymerase chain reaction

Abstract

This study aimed to evaluate the effect of inoculation with lyophilised and/or activated Lactobacillus buchneri on lactic acid bacteria populations in corn silage. Experimental treatments consisted of corn silage without additives or silage with the inoculants of L. buchneri (1 x 105 cfu/g) applied according to the manufacturer’s recommendations (1 g/tonne fodder) in the forms of the lyophilised inoculant and pre-activated inoculant. Purified isolates from corn silage with and without the inoculant were identified, and 93% of the isolates corresponded to the lactic acid bacteria of the species Lactobacillus plantarum. Among the isolates, no bacteria of the species L. buchneri were detected. The application of lyophilised or activated L. buchneri improved the microbiological profile and reduced ethanol production in corn silage, even without being identified among the isolates captured 70 days after ensilage.

Highlights:

  • Lactic acid bacteria showed greater development at 7 days of fermentation.
  • Lactobacillus plantarum predominated at 70 days, representing 93% of the total LAB population.
  • Lactobacillus buchneri improved its microbiological profile with decreased ethanol production.

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Published

05-07-2023

Issue

Vol. 55 No. 1 (2023): January-June

Section

Agroindustrial technologies

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Copyright (c) 2018 Revista de la Facultad de Ciencias Agrarias UNCuyo

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